Inhibition of protein synthesis by a toxic lectin from Viscum album L. (mistletoe)

Inhibition of protein synthesis by a toxic lectin from Viscum album L. (mistletoe).

1. The haemagglutinating and toxic lectin from Viscum album L. (mistletoe) inhibits protein synthesis in a lysate of rabbit reticulocytes, with an ID50 (concentration giving 50% inhibition) of 2.6 microgram/ml. This effect is enhanced (ID50 0.21 microgram/ml) if the lectin is reduced with 2-mercaptoethanol. 2. The lectin inhibits protein synthesis also in BL8L cells in culture. Inhibition occur...

Lectin from Viscum album L. inhibits protein synthesis in a lysate of rabbit reticulocytes

Mistletoe has been used as a drug against different diseases in po­ pular medicine for a long time, but the systematic research into anti-neoplastic activity of mistletoe extracts started only recently. Biological and anti-tumor activity of many protein fractions from mistletoe ex­ tract has been thouroughly investigated by V e s t e r et al. (1968 a, b), who determined optimal conditions for t...

Antioxidant Activity of European Mistletoe (Viscum album)

Effect of gamma irradiation on mistletoe (Viscum album) lectin-mediated toxicity and immunomodulatory activity☆

This study evaluated the effect of gamma irradiation on the reduction of the toxicity of mistletoe lectin using both in vitro and in vivo models. To extract the lectin from mistletoe, an (NH4)2SO4 precipitation method was employed and the precipitant purified using a Sepharose 4B column to obtain the pure lectin fraction. Purified lectin was then gamma-irradiated at doses of 0, 5, 10, 15, and 2...

Isolation and properties of three lectins from mistletoe (Viscum album L.).

Three lectins have been isolated from an extract of mistletoe (Viscum album) by affinity chromatography on partially hydrolysed Sepharose and human immunoglobulin- Sepharose. The lectins differ in molecular weight and sugar specificity (lectin I, mol.wt. 11500, D-galactose-specific; lectin II, mol.wt. 60000, both D-galactose- and N-acetyl-D-galactosamine-specific; lectin III, mol. wt. 50000, N-...